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Addgene

pBEL2057
(Plasmid #162145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162145 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD-His
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MlaFEDCB
  • Species
    E. coli
  • Mutation
    MlaF-(6xHis-MlaE, Tyr81Bpa)-MlaD-MlaC-MlaB

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEL2057 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 162145 ; http://n2t.net/addgene:162145 ; RRID:Addgene_162145)

  • For your References section:

    Structure of bacterial phospholipid transporter MlaFEDB with substrate bound. Coudray N, Isom GL, MacRae MR, Saiduddin MN, Bhabha G, Ekiert DC. Elife. 2020 Nov 25;9. pii: 62518. doi: 10.7554/eLife.62518. 10.7554/eLife.62518 PubMed 33236984
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