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pLenti6-AMPK alpha1 (1-312)
(Plasmid #162131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162131 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6/V5-Dest
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AMPK alpha1 (1-312)
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    PRKAA1 (a.k.a. AMPK, AMPK alpha 1, AMPKa1)
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6-AMPK alpha1 (1-312) was a gift from Boyi Gan (Addgene plasmid # 162131 ; http://n2t.net/addgene:162131 ; RRID:Addgene_162131)

  • For your References section:

    LncRNA NBR2 engages a metabolic checkpoint by regulating AMPK under energy stress. Liu X, Xiao ZD, Han L, Zhang J, Lee SW, Wang W, Lee H, Zhuang L, Chen J, Lin HK, Wang J, Liang H, Gan B. Nat Cell Biol. 2016 Apr;18(4):431-42. doi: 10.1038/ncb3328. Epub 2016 Mar 21. 10.1038/ncb3328 PubMed 26999735
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