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pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1
(Plasmid #162098)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162098 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Backbone manufacturer
    Vectorbuilder
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Puromycin resistant; EGFP linked to HA-FLAG-AU1
  • Alt name
    G-3-HFA
  • Species
    Synthetic
  • Mutation
    WT
  • Promoter EF1A
  • Tag / Fusion Protein
    • HA-FLAG-AU1 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

#20 out of 20 EGFP tagged combinations . Please visit https://doi.org/10.1101/2021.06.29.449991 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1 was a gift from Julien Sage (Addgene plasmid # 162098 ; http://n2t.net/addgene:162098 ; RRID:Addgene_162098)

  • For your References section:

    Spatial epitope barcoding reveals clonal tumor patch behaviors. Rovira-Clave X, Drainas AP, Jiang S, Bai Y, Baron M, Zhu B, Dallas AE, Lee MC, Chu TP, Holzem A, Ayyagari R, Bhattacharya D, McCaffrey EF, Greenwald NF, Markovic M, Coles GL, Angelo M, Bassik MC, Sage J, Nolan GP. Cancer Cell. 2022 Nov 14;40(11):1423-1439.e11. doi: 10.1016/j.ccell.2022.09.014. Epub 2022 Oct 13. 10.1016/j.ccell.2022.09.014 PubMed 36240778
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