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Addgene

pBScMXT + egl2
(Plasmid #16209)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16209 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBScMXT
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Xenopus Oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    K+-channel KCNQ
  • Alt name
    neag
  • Alt name
    F16B3.1
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    3200
  • Mutation
    N/A
  • GenBank ID
    NM_071001
  • Entrez Gene
    egl-2 (a.k.a. CELE_F16B3.1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Spe1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBScMXT + egl2 was a gift from Larry Salkoff (Addgene plasmid # 16209 ; http://n2t.net/addgene:16209 ; RRID:Addgene_16209)

  • For your References section:

    Block of an ether-a-go-go-like K(+) channel by imipramine rescues egl-2 excitation defects in Caenorhabditis elegans. Weinshenker D, Wei A, Salkoff L, Thomas JH. J Neurosci. 1999 Nov 15. 19(22):9831-40. PubMed 10559392
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