pGEX2TK-PDZ(HtrA2)
(Plasmid
#16207)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2TK
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Backbone manufacturerPharmacia
- Backbone size w/o insert (bp) 4950
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHtrA2
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Alt nameOmi
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SpeciesH. sapiens (human)
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Insert Size (bp)320
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Mutationamino acids 350–459
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GenBank IDNM_013247
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Entrez GeneHTRA2 (a.k.a. MGCA8, OMI, PARK13, PRSS25)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX 5' Sequencing primer
- 3′ sequencing primer pGEX 3' Sequencing primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX2TK-PDZ(HtrA2) was a gift from L. Miguel Martins (Addgene plasmid # 16207 ; http://n2t.net/addgene:16207 ; RRID:Addgene_16207) -
For your References section:
Binding specificity and regulation of the serine protease and PDZ domains of HtrA2/Omi. Martins LM, Turk BE, Cowling V, Borg A, Jarrell ET, Cantley LC, Downward J. J Biol Chem. 2003 Dec 5. 278(49):49417-27. 10.1074/jbc.M308659200 PubMed 14512424