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Addgene

pGEX2TK-PDZ(HtrA2)
(Plasmid #16207)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16207 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-2TK
  • Backbone manufacturer
    Pharmacia
  • Backbone size w/o insert (bp) 4950
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HtrA2
  • Alt name
    Omi
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    320
  • Mutation
    amino acids 350–459
  • GenBank ID
    NM_013247
  • Entrez Gene
    HTRA2 (a.k.a. MGCA8, OMI, PARK13, PRSS25)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX 5' Sequencing primer
  • 3′ sequencing primer pGEX 3' Sequencing primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX2TK-PDZ(HtrA2) was a gift from L. Miguel Martins (Addgene plasmid # 16207 ; http://n2t.net/addgene:16207 ; RRID:Addgene_16207)
  • For your References section:

    Binding specificity and regulation of the serine protease and PDZ domains of HtrA2/Omi. Martins LM, Turk BE, Cowling V, Borg A, Jarrell ET, Cantley LC, Downward J. J Biol Chem. 2003 Dec 5. 278(49):49417-27. 10.1074/jbc.M308659200 PubMed 14512424