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Addgene

LR (bact: HyPer)
(Plasmid #162069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDestTol2pA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7903
  • Total vector size (bp) 13128
  • Vector type
    Gateway Donor Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta actin 2
  • Alt name
    bactin2
  • Species
    D. rerio (zebrafish)
  • GenBank ID
    57935
  • Entrez Gene
    actb2 (a.k.a. actbb, bact2, bactin2)
  • Promoter T3
  • Tag / Fusion Protein
    • HyPer (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LR (bact: HyPer) was a gift from Philipp Niethammer (Addgene plasmid # 162069 ; http://n2t.net/addgene:162069 ; RRID:Addgene_162069)

  • For your References section:

    Tissue damage detection by osmotic surveillance. Enyedi B, Kala S, Nikolich-Zugich T, Niethammer P. Nat Cell Biol. 2013 Sep;15(9):1123-30. doi: 10.1038/ncb2818. Epub 2013 Aug 11. 10.1038/ncb2818 PubMed 23934216
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