pCS2+cPLA2-mKate2
(Plasmid #162068)

• Purpose: Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.
• Depositing Lab: Philipp Niethammer
• Publication: Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. doi: 10.1038/ncb2818. Epub 2013 Aug 11.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4085
• Total vector size (bp): 7047
• Vector type: Mammalian Expression ; Avian, Xenopus, Zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cytosolic phospholipase a2
• Alt name: cPLA2
• Alt name: pla2g4aa
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2961
• GenBank ID: 30554
• Entrez Gene: pla2g4aa (a.k.a. PLA2G4A, cpla2, pla2g4)
• Promoter: CMV
• Tag / Fusion Protein:
  • mKate2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pcs2+pla2-mk2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry