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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16205 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCVpuro
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 6300
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBRIT1
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SpeciesH. sapiens (human)
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Entrez GeneMCPH1 (a.k.a. BRIT1, MCT)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCVpuro BRIT1 was a gift from Shiaw-Yih Lin (Addgene plasmid # 16205 ; http://n2t.net/addgene:16205 ; RRID:Addgene_16205) -
For your References section:
BRIT1 regulates early DNA damage response, chromosomal integrity, and cancer. Rai R, Dai H, Multani AS, Li K, Chin K, Gray J, Lahad JP, Liang J, Mills GB, Meric-Bernstam F, Lin SY. Cancer Cell. 2006 Aug . 10(2):145-57. 10.1016/j.ccr.2006.07.002 PubMed 16872911