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PurposeBackground-free mRNA labeling bipartite superfolder GFP system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162038 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep323
- Backbone size w/o insert (bp) 7826
- Total vector size (bp) 9509
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMCP-GFP1-10 P2A PCP-GFP11
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Insert Size (bp)1683
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MCP-GFP1-10 P2A PCP-GFP11 was a gift from Hye Yoon Park (Addgene plasmid # 162038 ; http://n2t.net/addgene:162038 ; RRID:Addgene_162038) -
For your References section:
Live-cell imaging of single mRNA dynamics using split superfolder green fluorescent proteins with minimal background. Park SY, Moon HC, Park HY. RNA. 2020 Jan;26(1):101-109. doi: 10.1261/rna.067835.118. Epub 2019 Oct 22. 10.1261/rna.067835.118 PubMed 31641028