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Addgene

pLEX303-GFP-IQSEC1 v2N/v4C-WT
(Plasmid #162022)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162022 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEX303-GFP
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IQSEC1
  • Species
    H. sapiens (human)
  • Mutation
    v2N/v4C
  • Entrez Gene
    IQSEC1 (a.k.a. ARF-GEP100, ARFGEP100, BRAG2, GEP100)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/851410 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX303-GFP-IQSEC1 v2N/v4C-WT was a gift from David Bryant (Addgene plasmid # 162022 ; http://n2t.net/addgene:162022 ; RRID:Addgene_162022)

  • For your References section:

    An ARF GTPase module promoting invasion and metastasis through regulating phosphoinositide metabolism. Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L, Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth K, Bryant DM. Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4. 10.1038/s41467-021-21847-4 PubMed 33712589
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