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Addgene

pOX + nSlo2
(Plasmid #16202)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16202 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOX
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Xenopus Oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Caenorhabditis elegans SLOwpoke potassium channel
  • Alt name
    nSlo2
  • Alt name
    F08B12.3
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    3500
  • Mutation
    N/A
  • GenBank ID
    NM_077508
  • Entrez Gene
    slo-2 (a.k.a. CELE_F08B12.3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Xbo1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOX + nSlo2 was a gift from Larry Salkoff (Addgene plasmid # 16202 ; http://n2t.net/addgene:16202 ; RRID:Addgene_16202)

  • For your References section:

    SLO-2, a K+ channel with an unusual Cl- dependence. Yuan A, Dourado M, Butler A, Walton N, Wei A, Salkoff L. Nat Neurosci. 2000 Aug . 3(8):771-9. 10.1038/77670 PubMed 10903569

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