pLEX304-GFP-v2 IQSEC1 WT
(Plasmid
#162017)
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PurposeExpression of IQSEC1 splice variant 2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162017 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLEX304-GFP
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIQSEC1
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SpeciesH. sapiens (human)
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Entrez GeneIQSEC1 (a.k.a. ARF-GEP100, ARFGEP100, BRAG2, GEP100)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/851410 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX304-GFP-v2 IQSEC1 WT was a gift from David Bryant (Addgene plasmid # 162017 ; http://n2t.net/addgene:162017 ; RRID:Addgene_162017) -
For your References section:
An ARF GTPase module promoting invasion and metastasis through regulating phosphoinositide metabolism. Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L, Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth K, Bryant DM. Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4. 10.1038/s41467-021-21847-4 PubMed 33712589