pLKO.1 Puro shRNA SHIP1 KD2
(Plasmid
#162013)
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PurposeSHIP1 shRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162013 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLKO.1
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameINPP5D
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Alt nameSHIP1
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gRNA/shRNA sequenceUnspecified
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SpeciesCanis Lupus
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Entrez GeneINPP5D
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO.1 Puro shRNA SHIP1 KD2 was a gift from David Bryant (Addgene plasmid # 162013 ; http://n2t.net/addgene:162013 ; RRID:Addgene_162013) -
For your References section:
The phospholipid PI(3,4)P2 is an apical identity determinant. Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, McGarry L, Shanks E, Mostov KE, Bryant DM. Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8. 10.1038/s41467-018-07464-8 PubMed 30487552