pRevTRE SGFP2 Inpp5E WT shRNA resistant
(Plasmid
#161997)
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PurposeTet-responsive retroviral expression vector. Contains INPP5E. Also contains SGFP tag.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161997 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRevTRE
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Vector typeRetroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameINPP5E
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SpeciesH. sapiens (human)
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Entrez GeneINPP5E (a.k.a. CORS1, CPD4, JBTS1, MORMS, PPI5PIV, pharbin)
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Tag
/ Fusion Protein
- SGFP (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRevTRE SGFP2 Inpp5E WT shRNA resistant was a gift from David Bryant (Addgene plasmid # 161997 ; http://n2t.net/addgene:161997 ; RRID:Addgene_161997) -
For your References section:
The phospholipid PI(3,4)P2 is an apical identity determinant. Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, McGarry L, Shanks E, Mostov KE, Bryant DM. Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8. 10.1038/s41467-018-07464-8 PubMed 30487552
