mCherry-C1 SNX9 RYK mutant R286A Y287A K288A
(Plasmid
#161993)
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PurposeMammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161993 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepmCherry-C1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNX9
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SpeciesM. musculus (mouse)
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MutationR286A Y287A K288A
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Entrez GeneSnx9 (a.k.a. 2700073N08Rik, SD, SDP1, SH3P, SH3PX1)
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Tag
/ Fusion Protein
- mCherry (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-C1 SNX9 RYK mutant R286A Y287A K288A was a gift from David Bryant (Addgene plasmid # 161993 ; http://n2t.net/addgene:161993 ; RRID:Addgene_161993) -
For your References section:
The phospholipid PI(3,4)P2 is an apical identity determinant. Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, McGarry L, Shanks E, Mostov KE, Bryant DM. Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8. 10.1038/s41467-018-07464-8 PubMed 30487552
