GFP-2xPH-TAPP1 R211L mutant
(Plasmid
#161991)
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PurposeExpresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161991 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneeGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePH-TAPP1
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Alt namePLEKHA1
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SpeciesH. sapiens (human)
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MutationR211L
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Entrez GenePLEKHA1 (a.k.a. TAPP1)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-2xPH-TAPP1 R211L mutant was a gift from David Bryant (Addgene plasmid # 161991 ; http://n2t.net/addgene:161991 ; RRID:Addgene_161991) -
For your References section:
The phospholipid PI(3,4)P2 is an apical identity determinant. Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, McGarry L, Shanks E, Mostov KE, Bryant DM. Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8. 10.1038/s41467-018-07464-8 PubMed 30487552
