GFP-PIK3C2B
(Plasmid #161989)

• Purpose: Mammalian expression vector containing PIK3C2B tagged with GFP
• Depositing Labs: David Bryant, Volker Haucke
• Publication: Marat et al Science. 2017 Jun 2;356(6341):968-972. doi: 10.1126/science.aaf8310.

Backbone

• Vector backbone: eGFP-C1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PIK3C2B
• Alt name: accession number CAA74194
• Species: H. sapiens (human)
• Mutation: siRNA resistance to siRNA#2 with 4 silent mutations: 5’- GCTACCAGTTACGAGGACT-3’.
• Entrez Gene: PIK3C2B (a.k.a. C2-PI3K)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has also been described in: Wallroth, A., Koch, P.A., Marat, A.L., Krause, E., Haucke, V. (2019) Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signaling via mTORC1. Nature Cell Biol. 21, 1093-1101. doi: 10.1038/s41556-019-0377-3