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Addgene

pMSR0
(Plasmid #161984)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161984 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMSR0
  • Backbone size (bp) 6696
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGTTTTGTAAACGAATTGC
  • 3′ sequencing primer CTCACGTTAAGGGATTTTG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note Addgene NGS result identified a mismatch in pBP1_RepA. Depositor has not confirmed if this is a concern for plasmid function. We recommend additional QC to confirm plasmid is functional.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSR0 was a gift from Olga Soutourina (Addgene plasmid # 161984 ; http://n2t.net/addgene:161984 ; RRID:Addgene_161984)
  • For your References section:

    Type I toxin-antitoxin systems contribute to the maintenance of mobile genetic elements in Clostridioides difficile. Peltier J, Hamiot A, Garneau JR, Boudry P, Maikova A, Hajnsdorf E, Fortier LC, Dupuy B, Soutourina O. Commun Biol. 2020 Nov 27;3(1):718. doi: 10.1038/s42003-020-01448-5. 10.1038/s42003-020-01448-5 PubMed 33247281