pMSR0
(Plasmid
#161984)
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Purpose(Empty Backbone) Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 161984 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMSR0
- Backbone size (bp) 6696
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGTTTTGTAAACGAATTGC
- 3′ sequencing primer CTCACGTTAAGGGATTTTG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note Addgene NGS result identified a mismatch in pBP1_RepA. Depositor has not confirmed if this is a concern for plasmid function. We recommend additional QC to confirm plasmid is functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSR0 was a gift from Olga Soutourina (Addgene plasmid # 161984 ; http://n2t.net/addgene:161984 ; RRID:Addgene_161984) -
For your References section:
Type I toxin-antitoxin systems contribute to the maintenance of mobile genetic elements in Clostridioides difficile. Peltier J, Hamiot A, Garneau JR, Boudry P, Maikova A, Hajnsdorf E, Fortier LC, Dupuy B, Soutourina O. Commun Biol. 2020 Nov 27;3(1):718. doi: 10.1038/s42003-020-01448-5. 10.1038/s42003-020-01448-5 PubMed 33247281