pMSR0
(Plasmid #161984)

• Purpose: (Empty Backbone) Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker
• Depositing Lab: Olga Soutourina
• Publication: Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi: 10.1038/s42003-020-01448-5.

Backbone

• Vector backbone: pMSR0
• Backbone size (bp): 6696
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGTTTTGTAAACGAATTGC
• 3′ sequencing primer: CTCACGTTAAGGGATTTTG

Resource Information

• Supplemental Documents:
  • pMSR0.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note Addgene NGS result identified a mismatch in pBP1_RepA. Depositor has not confirmed if this is a concern for plasmid function. We recommend additional QC to confirm plasmid is functional.