pMSR
(Plasmid
#161983)
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Purpose(Empty Backbone) Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection marker
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161983 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSR
- Backbone size (bp) 5624
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGTTTTGTAAACGAATTGC
- 3′ sequencing primer CTCACGTTAAGGGATTTTG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSR was a gift from Olga Soutourina (Addgene plasmid # 161983 ; http://n2t.net/addgene:161983 ; RRID:Addgene_161983) -
For your References section:
Type I toxin-antitoxin systems contribute to the maintenance of mobile genetic elements in Clostridioides difficile. Peltier J, Hamiot A, Garneau JR, Boudry P, Maikova A, Hajnsdorf E, Fortier LC, Dupuy B, Soutourina O. Commun Biol. 2020 Nov 27;3(1):718. doi: 10.1038/s42003-020-01448-5. 10.1038/s42003-020-01448-5 PubMed 33247281
Map uploaded by the depositor.
