pBSc polyA + Shal1
(Plasmid
#16196)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16196 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBSc
- Backbone size w/o insert (bp) 3200
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Vector typeXenopus Oocyte expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDrosophila melanogaster Shaker cognate l
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Alt namedShal
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Alt nameCG9262-PB
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Alt nameisoform B
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SpeciesD. melanogaster (fly)
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Insert Size (bp)2000
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MutationN/A
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GenBank IDNM_079435
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Entrez GeneShal (a.k.a. Dmel_CG9262, CG9262, DNK[[v]]4, Dmel\CG9262, K[[v]]4, Kv4, Kv4/Shal, Sh-l, ShaI, Shl, dShal, shaI, shal)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind111 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSc polyA + Shal1 was a gift from Larry Salkoff (Addgene plasmid # 16196 ; http://n2t.net/addgene:16196 ; RRID:Addgene_16196) -
For your References section:
K+ current diversity is produced by an extended gene family conserved in Drosophila and mouse. Wei A, Covarrubias M, Butler A, Baker K, Pak M, Salkoff L. Science. 1990 May 4. 248(4955):599-603. 10.1126/science.2333511 PubMed 2333511
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/41/89/effc4882-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)