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Addgene

pBSc polyA + Shal1
(Plasmid #16196)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSc
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Xenopus Oocyte expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Drosophila melanogaster Shaker cognate l
  • Alt name
    dShal
  • Alt name
    CG9262-PB
  • Alt name
    isoform B
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2000
  • Mutation
    N/A
  • GenBank ID
    NM_079435
  • Entrez Gene
    Shal (a.k.a. Dmel_CG9262, CG9262, DNK[[v]]4, Dmel\CG9262, K[[v]]4, Kv4, Kv4/Shal, Sh-l, ShaI, Shl, dShal, shaI, shal)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind111 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSc polyA + Shal1 was a gift from Larry Salkoff (Addgene plasmid # 16196 ; http://n2t.net/addgene:16196 ; RRID:Addgene_16196)

  • For your References section:

    K+ current diversity is produced by an extended gene family conserved in Drosophila and mouse. Wei A, Covarrubias M, Butler A, Baker K, Pak M, Salkoff L. Science. 1990 May 4. 248(4955):599-603. 10.1126/science.2333511 PubMed 2333511
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