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Addgene

pCGFP-BC
(Plasmid #161959)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET25b
  • Backbone manufacturer
    Novagen
  • Modifications to backbone
    Added His8, Thrombin recognition site, GFPuv (modified- alanine 206 to lysine, first methionine mutated to valine) removed XhoI, BamHI, HindIII, NcoI sites). Modified MCS- NcoI site knocked out, NdeI site converted to new NcoI site.
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tag / Fusion Protein
    • GFPuv His8

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was developed while Eric Gouaux was a Professor at Columbia University.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCGFP-BC was a gift from Eric Gouaux (Addgene plasmid # 161959 ; http://n2t.net/addgene:161959 ; RRID:Addgene_161959)

  • For your References section:

    Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins. Kawate T, Gouaux E. Structure. 2006 Apr;14(4):673-81. doi: 10.1016/j.str.2006.01.013. 10.1016/j.str.2006.01.013 PubMed 16615909
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