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Addgene

IBMc396
(Plasmid #161954)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161954 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA321
  • Backbone manufacturer
    The SEVA Commonwealth
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
  • Species
    Synthetic
  • Promoter PBAD

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer PS1 - AGGGCGGCGGATTTGTCC
  • 3′ sequencing primer PS2 - GCGGCAACCGAGCGTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IBMc396 was a gift from Baojun Wang (Addgene plasmid # 161954 ; http://n2t.net/addgene:161954 ; RRID:Addgene_161954)

  • For your References section:

    A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction. Ho TYH, Shao A, Lu Z, Savilahti H, Menolascina F, Wang L, Dalchau N, Wang B. Nat Commun. 2021 Apr 13;12(1):2200. doi: 10.1038/s41467-021-22404-9. 10.1038/s41467-021-22404-9 PubMed 33850130
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