pBScMXT + Slo1
(Plasmid #16194)

• Depositing Lab: Larry Salkoff
• Publication: McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H767-77.

Backbone

• Vector backbone: pBScMXT
• Backbone size w/o insert (bp): 3200
• Vector type: Xenopus Oocyte expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: calcium-activated potassium channel
• Alt name: AAA92290
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 3900
• Mutation: N/A
• GenBank ID: U23767
• Entrez Gene: KCNMA1 (a.k.a. BKTM, CADEDS, IEG16, KCa1.1, LIWAS, MaxiK, PNKD3, SAKCA, SLO, SLO-ALPHA, SLO1, bA205K10.1, hSlo, mSLO1)
• Entrez Gene: Kcnma1 (a.k.a. 5730414M22Rik, BKCA alpha, BKCa, KCa1.1, MaxiK, Slo, Slo1, k(VCA)alpha, mSlo, mSlo1, slo-alpha)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Cla1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results do not exactly match the above GenBank accession numbers U23767 (nucleotide) and AAA92290 (protein) due to the cloning strategy employed for this plasmid. The beginning of the insert sequence from the Hind3 site (AAGCTT) to the Mlu1 site (ACGCGT) is from a mouse cDNA that contains an N-terminus that is highly conserved but not identical to that of the human channel. Prior to the starting Met of Slo1, this plasmid contains a short sequence from the mouse Slo1 5' untranslated region (5'UTR).