IBMc090
(Plasmid
#161937)
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PurposeCarries engineered transposon for IBM
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 161937 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTHSSd_1
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Backbone manufacturerChristopher Voigt's Lab
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byModified from #59947 in Addgene
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IBMc090 was a gift from Baojun Wang (Addgene plasmid # 161937 ; http://n2t.net/addgene:161937 ; RRID:Addgene_161937) -
For your References section:
A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction. Ho TYH, Shao A, Lu Z, Savilahti H, Menolascina F, Wang L, Dalchau N, Wang B. Nat Commun. 2021 Apr 13;12(1):2200. doi: 10.1038/s41467-021-22404-9. 10.1038/s41467-021-22404-9 PubMed 33850130