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Addgene

pET28c-Ku
(Plasmid #161936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28c
  • Backbone size w/o insert (bp) 5238
  • Total vector size (bp) 6078
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ku
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
    840
  • Entrez Gene
    mku (a.k.a. Rv0937c)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GATGTCGGCGATATAGGC
  • 3′ sequencing primer GCAGCCAACTCAGCTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28c-Ku was a gift from Harris Wang (Addgene plasmid # 161936 ; http://n2t.net/addgene:161936 ; RRID:Addgene_161936)

  • For your References section:

    Protecting Linear DNA Templates in Cell-Free Expression Systems from Diverse Bacteria. Yim SS, Johns NI, Noireaux V, Wang HH. ACS Synth Biol. 2020 Sep 29. doi: 10.1021/acssynbio.0c00277. 10.1021/acssynbio.0c00277 PubMed 32926785
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