pEC1.2/EC1.1_spCas9_GFP
(Plasmid
#161933)
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Purpose(Empty Backbone) CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 161933 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHEE401E
- Backbone size (bp) 16255
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Vector typeCRISPR
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Selectable markersGFP seed selection
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byQi-Jun Chen, State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEC1.2/EC1.1_spCas9_GFP was a gift from Christian Fankhauser (Addgene plasmid # 161933 ; http://n2t.net/addgene:161933 ; RRID:Addgene_161933)