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pJT040 lenti MCS pEF mIFP WPRE
(Plasmid #161929)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161929 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMCB320
  • Backbone size w/o insert (bp) 8907
  • Total vector size (bp) 8047
  • Modifications to backbone
    Replace mCherry Puro with mIFP. Replace U6 sgRNA with MCS.
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mIFP
  • Promoter pEF

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Lentiviral vector for expression of mIFP. Has a multiple cloning site upstream of the pEF-mIFP cassette.

Cloned with reagents shared by and in collaboration with Michael Bassik.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT040 lenti MCS pEF mIFP WPRE was a gift from Michael Bassik & Lacra Bintu (Addgene plasmid # 161929 ; http://n2t.net/addgene:161929 ; RRID:Addgene_161929)

  • For your References section:

    High-Throughput Discovery and Characterization of Human Transcriptional Effectors. Tycko J, DelRosso N, Hess GT, Aradhana, Banerjee A, Mukund A, Van MV, Ego BK, Yao D, Spees K, Suzuki P, Marinov GK, Kundaje A, Bassik MC, Bintu L. Cell. 2020 Dec 9. pii: S0092-8674(20)31541-5. doi: 10.1016/j.cell.2020.11.024. 10.1016/j.cell.2020.11.024 PubMed 33326746
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