pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
(Plasmid
#161928)
-
PurposeDonor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
-
Depositing Labs
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepAAVS1 donor
-
Modifications to backboneReplace mCherry Puro with mIFP. Replace U6 sgRNA with MCS.
-
Vector typeMammalian Expression, Synthetic Biology
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSurface marker and Citrine activation reporter
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned with reagents shared by and in collaboration with Michael Bassik.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA was a gift from Michael Bassik & Lacra Bintu (Addgene plasmid # 161928 ; http://n2t.net/addgene:161928 ; RRID:Addgene_161928) -
For your References section:
High-Throughput Discovery and Characterization of Human Transcriptional Effectors. Tycko J, DelRosso N, Hess GT, Aradhana, Banerjee A, Mukund A, Van MV, Ego BK, Yao D, Spees K, Suzuki P, Marinov GK, Kundaje A, Bassik MC, Bintu L. Cell. 2020 Dec 9. pii: S0092-8674(20)31541-5. doi: 10.1016/j.cell.2020.11.024. 10.1016/j.cell.2020.11.024 PubMed 33326746
