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pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE
(Plasmid #161925)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161925 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJT040
  • Backbone size (bp) 8000
  • Modifications to backbone
    replace mIFP with rTetR-LibCloneSite-T2A-mCherry-BSD
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Promoter pEF
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone plasmid for cloning individual effectors or libraries of effector domains using GoldenGate cloning with BsmBI.

Cloned with reagents shared by and in collaboration with Michael Bassik.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE was a gift from Michael Bassik & Lacra Bintu (Addgene plasmid # 161925 ; http://n2t.net/addgene:161925 ; RRID:Addgene_161925)

  • For your References section:

    High-Throughput Discovery and Characterization of Human Transcriptional Effectors. Tycko J, DelRosso N, Hess GT, Aradhana, Banerjee A, Mukund A, Van MV, Ego BK, Yao D, Spees K, Suzuki P, Marinov GK, Kundaje A, Bassik MC, Bintu L. Cell. 2020 Dec 9. pii: S0092-8674(20)31541-5. doi: 10.1016/j.cell.2020.11.024. 10.1016/j.cell.2020.11.024 PubMed 33326746
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