pGEM-sema3B-3'-probe
(Plasmid
#16192)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16192 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T Easy
- Backbone size w/o insert (bp) 3015
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesemaphorin 3B
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SpeciesM. musculus (mouse)
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Insert Size (bp)407
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Entrez GeneSema3b (a.k.a. LUCA, Se, SemA, Semaa, sem, sema5, semaV)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a 3' probe for the sema3B targeting construct resulting in a shift of 19.8 kB to 7.9 kB in HindIII digested ES gDNA. The probe can be released from pGEM-T Easy by NotI or EcoRI digestion
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-sema3B-3'-probe was a gift from Peter Mombaerts (Addgene plasmid # 16192) -
For your References section:
Axonal wiring of guanylate cyclase-D-expressing olfactory neurons is dependent on neuropilin 2 and semaphorin 3F. Walz A, Feinstein P, Khan M, Mombaerts P. Development. 2007 Nov . 134(22):4063-72. 10.1242/dev.008722 PubMed 17942483