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pGEM-sema3F-5'-probe
(Plasmid #16190)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16190 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEM-T Easy
  • Backbone size w/o insert (bp) 3015
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    semaphorin 3F
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    404
  • Entrez Gene
    Sema3f (a.k.a. Sem, Sema4, Semak)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a 5' probe for the sema3F targeting construct resulting in a shift of 10.2 kB to 7.8 kB in EcoRI digested ES gDNA. The probe can be released from pGEM-T Easy by NotI or EcoRI digestion

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-sema3F-5'-probe was a gift from Peter Mombaerts (Addgene plasmid # 16190)

  • For your References section:

    Axonal wiring of guanylate cyclase-D-expressing olfactory neurons is dependent on neuropilin 2 and semaphorin 3F. Walz A, Feinstein P, Khan M, Mombaerts P. Development. 2007 Nov . 134(22):4063-72. 10.1242/dev.008722 PubMed 17942483
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