pCDNA3.3TOPO-T7-hCas9
(Plasmid
#161876)
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PurposeIn vitro transcription of SpCas9
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161876 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.3
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman codon optimized SpCas9
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer TK-pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGeorge Church
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.3TOPO-T7-hCas9 was a gift from Stephane Pelletier (Addgene plasmid # 161876 ; http://n2t.net/addgene:161876 ; RRID:Addgene_161876) -
For your References section:
Mouse genome engineering via CRISPR-Cas9 for study of immune function. Pelletier S, Gingras S, Green DR. Immunity. 2015 Jan 20;42(1):18-27. doi: 10.1016/j.immuni.2015.01.004. 10.1016/j.immuni.2015.01.004 PubMed 25607456
