pcDNA3-Rs4231-RBD-8h
(Plasmid #161824)

• Purpose: Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4231
• Depositing Lab: Erik Procko
• Publication: Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf1738. doi: 10.1126/sciadv.abf1738. Print 2021 Feb.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5332
• Total vector size (bp): 6034
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Receptor-binding domain (RBD) of spike protein S
• Alt name: RBD
• Species: Rs4231 bat coronavirus
• Insert Size (bp): 702
• Mutation: Human codon optimized RBD (a.a. T320-D518)
• GenBank ID: ATO98157.1
• Promoter: CMV
• Tags / Fusion Proteins:
  • GSG linker and 8his tag (C terminal on insert)
  • HA leader sequence (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-fwd
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry