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Addgene

pcDNA3-SARS-1-RBD-8h
(Plasmid #161821)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 161821 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5332
  • Total vector size (bp) 6034
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Receptor-binding domain (RBD) of spike protein S
  • Alt name
    RBD
  • Species
    SARS coronavirus 1 (Urbani isolate)
  • Insert Size (bp)
    702
  • Mutation
    Human codon optimized RBD (a.a. T320-D518)
  • GenBank ID
    AAP13441.1
  • Promoter CMV
  • Tags / Fusion Proteins
    • GSG linker and 8his tag (C terminal on insert)
    • HA leader sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-fwd
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-SARS-1-RBD-8h was a gift from Erik Procko (Addgene plasmid # 161821 ; http://n2t.net/addgene:161821 ; RRID:Addgene_161821)

  • For your References section:

    An engineered decoy receptor for SARS-CoV-2 broadly binds protein S sequence variants. Chan KK, Tan TJC, Narayanan KK, Procko E. Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf1738. doi: 10.1126/sciadv.abf1738. Print 2021 Feb. 10.1126/sciadv.abf1738 PubMed 33597251
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