pGEX4-4T-1 Acetobacter AGSC
(Plasmid
#161813)
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PurposeExpresses AGSC domain from Acetobacter type I SMODS/CD-NTase enzyme
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAGSC domain Acetobacter SMODS/CD-NTase enzyme
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SpeciesAcetobacter
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Insert Size (bp)456
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Tags
/ Fusion Proteins
- TEV (N terminal on insert)
- His (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer 5GEX
- 3′ sequencing primer 3GEX (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX4-4T-1 Acetobacter AGSC was a gift from Douglas Feldman (Addgene plasmid # 161813 ; http://n2t.net/addgene:161813 ; RRID:Addgene_161813)
