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pTIN-ura4
(Plasmid #161808)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161808 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDS472a
  • Vector type
    Yeast Expression
  • Promoter Pho1
  • Selectable markers
    S. pombe ura4

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Angad Garg, Beate Schwer, Susan Forsburg

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Adapted from pTIN, described in Garg A. A lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeast Schizosaccharomyces pombe. RNA. 2020 Aug 11:rna.076000.120. doi: 10.1261/rna.076000.120. Epub ahead of print. PMID: 32788323.

MCS - NotI / PstI / XhoI / XmaI / SmaI

Compatible for co-transformation with pTIN

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTIN-ura4 was a gift from Stewart Shuman (Addgene plasmid # 161808 ; http://n2t.net/addgene:161808 ; RRID:Addgene_161808)
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