pCMV-monSTIM1
(Plasmid #161795)

• Purpose: Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)
• Depositing Lab: Won Do Heo
• Publication: Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi: 10.1038/s41467-019-14005-4.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3950
• Total vector size (bp): 7631
• Modifications to backbone: EGFP gene was removed and re-inserted at restriction enzyme sites NheI and BamHI.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MonSTIM1
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Insert Size (bp): 3678
• Entrez Gene: STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-pAR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry