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Addgene

Plenti-ARE-HSVTK-luc
(Plasmid #161789)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161789 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti-Luc-2A-Ctx
  • Backbone manufacturer
    Systems Biosciences
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 10962
  • Modifications to backbone
    Added ARE (Murine gclc promoter sequnce) to make Luc-2A-Ctx NRF2 dependent and mCherry/Blasticidin for selection
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers
    Blasticidin ; mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Luciferase-T2A-HSV-TK
  • Species
    Synthetic
  • GenBank ID
    N/A
  • Promoter Murine Gclc ARE

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    mCherry P2A blasticidin
  • Species
    Synthetic
  • GenBank ID
    N/A
  • Promoter EF1a

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Plenti-ARE-HSVTK-luc was a gift from Hans-Guido Wendel (Addgene plasmid # 161789)

  • For your References section:

    The Oncogenic Action of NRF2 Depends on De-glycation by Fructosamine-3-Kinase. Sanghvi VR, Leibold J, Mina M, Mohan P, Berishaj M, Li Z, Miele MM, Lailler N, Zhao C, de Stanchina E, Viale A, Akkari L, Lowe SW, Ciriello G, Hendrickson RC, Wendel HG. Cell. 2019 Aug 8;178(4):807-819.e21. doi: 10.1016/j.cell.2019.07.031. 10.1016/j.cell.2019.07.031 PubMed 31398338
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