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Addgene

pEE22
(Plasmid #161787)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161787 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSW23T
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 4440
  • Vector type
    Conjugative plasmid to deliver Hi-SC2 reporter

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    FCV14
  • Growth instructions
    Please see depositor comments at bottom of page
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hi-SC2 reporter
  • Species
    Synthetic
  • Insert Size (bp)
    1363
  • Mutation
    Himar site included MmeI site, tandem loxP sites, Rd2SP sequence for Illumina sequencing
  • Promoter n/a

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Strain FCV14 [E. coli DH5α producing the R6K π protein XerC::KanR]
Publication: https://pubmed.ncbi.nlm.nih.gov/31420511/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEE22 was a gift from François-Xavier Barre (Addgene plasmid # 161787 ; http://n2t.net/addgene:161787 ; RRID:Addgene_161787)

  • For your References section:

    High-Resolution Whole-Genome Analysis of Sister-Chromatid Contacts. Espinosa E, Paly E, Barre FX. Mol Cell. 2020 Sep 3;79(5):857-869.e3. doi: 10.1016/j.molcel.2020.06.033. Epub 2020 Jul 17. 10.1016/j.molcel.2020.06.033 PubMed 32681820
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