pEE22
(Plasmid #161787)

• Purpose: Hi-SC2 reporter delivery plasmid
• Depositing Lab: François-Xavier Barre
• Publication: Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. doi: 10.1016/j.molcel.2020.06.033. Epub 2020 Jul 17.

Backbone

• Vector backbone: pSW23T
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 4440
• Vector type: Conjugative plasmid to deliver Hi-SC2 reporter

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): FCV14
• Growth instructions: Please see depositor comments at bottom of page
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Hi-SC2 reporter
• Species: Synthetic
• Insert Size (bp): 1363
• Mutation: Himar site included MmeI site, tandem loxP sites, Rd2SP sequence for Illumina sequencing
• Promoter: n/a

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: various steps
• 3′ sequencing primer: various steps

Resource Information

• Supplemental Documents:
  • pEE22.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Strain FCV14 [E. coli DH5α producing the R6K π protein XerC::KanR]
Publication: https://pubmed.ncbi.nlm.nih.gov/31420511/