pSMALB
(Plasmid
#161786)
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Purpose(Empty Backbone) Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161786 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMA1
- Backbone size (bp) 10000
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Modifications to backboneInserted loxP and Gateway acceptor sites to facilitate insertion of transgene. Created bidirectional CMV-SFFV promotor for high expression of transgene and TagBFP.
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Vector typeMammalian Expression, Lentiviral
- Promoter Bidirectional minhCMV and SFFV
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Tag
/ Fusion Protein
- TagBFP
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSMALB was a gift from John Dick & Peter van Galen (Addgene plasmid # 161786 ; http://n2t.net/addgene:161786 ; RRID:Addgene_161786) -
For your References section:
Reduced lymphoid lineage priming promotes human hematopoietic stem cell expansion. van Galen P, Kreso A, Wienholds E, Laurenti E, Eppert K, Lechman ER, Mbong N, Hermans K, Dobson S, April C, Fan JB, Dick JE. Cell Stem Cell. 2014 Jan 2;14(1):94-106. doi: 10.1016/j.stem.2013.11.021. 10.1016/j.stem.2013.11.021 PubMed 24388174
Map uploaded by the depositor.
