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Addgene

pSMAL
(Plasmid #161785)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161785 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MA1
  • Backbone size (bp) 10000
  • Modifications to backbone
    Inserted loxP and Gateway acceptor sites to facilitate insertion of transgene. Created bidirectional CMV-SFFV promotor for high expression of transgene and eGFP.
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter Bidirectional minhCMV and SFFV

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSMAL was a gift from John Dick & Peter van Galen (Addgene plasmid # 161785 ; http://n2t.net/addgene:161785 ; RRID:Addgene_161785)

  • For your References section:

    Reduced lymphoid lineage priming promotes human hematopoietic stem cell expansion. van Galen P, Kreso A, Wienholds E, Laurenti E, Eppert K, Lechman ER, Mbong N, Hermans K, Dobson S, April C, Fan JB, Dick JE. Cell Stem Cell. 2014 Jan 2;14(1):94-106. doi: 10.1016/j.stem.2013.11.021. 10.1016/j.stem.2013.11.021 PubMed 24388174
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