pMALB
(Plasmid
#161784)
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Purpose(Empty Backbone) Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161784 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMA1
- Backbone size (bp) 10000
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Modifications to backboneInserted loxP and Gateway acceptor sites to drive transgene expression from the bidirectional promoter.
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Vector typeMammalian Expression, Lentiviral
- Promoter Bidirectional minhCMV and hPGK
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMALB was a gift from John Dick & Peter van Galen (Addgene plasmid # 161784 ; http://n2t.net/addgene:161784 ; RRID:Addgene_161784) -
For your References section:
Reduced lymphoid lineage priming promotes human hematopoietic stem cell expansion. van Galen P, Kreso A, Wienholds E, Laurenti E, Eppert K, Lechman ER, Mbong N, Hermans K, Dobson S, April C, Fan JB, Dick JE. Cell Stem Cell. 2014 Jan 2;14(1):94-106. doi: 10.1016/j.stem.2013.11.021. 10.1016/j.stem.2013.11.021 PubMed 24388174
Map uploaded by the depositor.
