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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR223
- Backbone size w/o insert (bp) 5005
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePoly(rC) binding protein 1
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Alt namePCBP1
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SpeciesH. sapiens (human)
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Insert Size (bp)1068
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Entrez GenePCBP1 (a.k.a. HEL-S-85, HNRPE1, HNRPX, hnRNP-E1, hnRNP-X)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attP1 (destroyed during cloning)
- 3′ cloning site attP2 (destroyed during cloning)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGATGCCGGTGTGACTGA-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GCTGCACCCCATGCCCTTC-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-PCBP1 was a gift from Huda Zoghbi (Addgene plasmid # 16177 ; http://n2t.net/addgene:16177 ; RRID:Addgene_16177) -
For your References section:
A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Lim J, Hao T, Shaw C, Patel AJ, Szabo G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabasi AL, Vidal M, Zoghbi HY. Cell. 2006 May 19. 125(4):801-14. 10.1016/j.cell.2006.03.032 PubMed 16713569