Ingolia Lab S. cerevisiae CRISPRi v1: Barcodes 181005
(Pooled Library #161769)
-
Purpose
This CRISPR inhibition library targets the yeast genome with Tet-inducible guide RNAs linked to 25-nucleotide barcodes.
-
Vector Backbone
pNTI661 pRPR1(TetO)-sgRNA (Plasmid #139475) - does not express Cas9
-
Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 161769 | Yeast CRISPRi v1 - Barcodes 181005 Library† | 1 | $430 | Add to Cart | |
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pNTI647 dCas9-Mxi1 TetR KanMX (Addgene #139474).
Library Details
-
SpeciesYeast
-
Genes targeted~6,700
-
gRNAs44,580
-
Controls344 no-gRNA barcodes
-
Selectable MarkerLEU2
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
-
Volume∼20 µL
-
Concentration50 ng/µL
Resource Information
-
Protocols
-
Depositor Data
-
Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositing lab has provided a web page with additional resources (Link opens in a new window) to support use of this library.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005 library was a gift from Nicholas Ingolia (Addgene #161769) -
For your References section:
A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast. McGlincy NJ, Meacham ZA, Swain K, Muller R, Baum R, Ingolia NT. BMC Genomics. 2021 Mar 23;22(1):205. doi: 10.1186/s12864-021-07518-0. PubMed 33757429 (Link opens in a new window)