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Addgene

pTRANS-Pho2-1/2-2-tRNA
(Plasmid #161762)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161762 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRANS_220
  • Backbone manufacturer
    Voytas Plant Genome Engineering Tool kit
  • Backbone size w/o insert (bp) 9500
  • Total vector size (bp) 17467
  • Vector type
    Plant Expression
  • Selectable markers
    neomycin phosphotransferase (kanR)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 and gRNAs
  • gRNA/shRNA sequence
    MsPho2-1 and MsPho2-2
  • Species
    alflafa
  • Promoter CmYLCV Promoter

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRANS-Pho2-1/2-2-tRNA was a gift from Shaun Curtin (Addgene plasmid # 161762 ; http://n2t.net/addgene:161762 ; RRID:Addgene_161762)

  • For your References section:

    Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate. Miller SS, Dornbusch MR, Farmer AD, Huertas R, Gutierrez-Gonzalez JJ, Young ND, Samac DA, Curtin SJ. G3 (Bethesda). 2022 May 30;12(6). pii: 6574359. doi: 10.1093/g3journal/jkac096. 10.1093/g3journal/jkac096 PubMed 35471600
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