pcDNA3-erExRai-AKAR2
(Plasmid #161756)

• Purpose: ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.
• Depositing Lab: Jin Zhang
• Publication: Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038/s41589-020-00660-y. doi: 10.1038/s41589-020-00660-y.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5517
• Total vector size (bp): 6822
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CYP450-ExRai-AKAR2
• Species: Synthetic
• Insert Size (bp): 1305
• Promoter: CMV
• Tag / Fusion Protein:
  • N-terminal 27 amino acids from CYP450 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-erExRai-AKAR2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry