GSX2-P2A-tGFP-DTA
(Plasmid #161750)

• Purpose: tGFP plasmid for the c-terminal targeting of human GSX2 locus
• Depositing Lab: Elena Cattaneo
• Publication: Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi: 10.1016/j.scr.2020.102016. Epub 2020 Oct 1.

Backbone

• Vector backbone: GSX2P2Adonor
• Backbone manufacturer: Origene
• Total vector size (bp): 8934
• Modifications to backbone: addition of a DTA selection cassette
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: turboGFP
• Species: copepod Pontellina plumata
• Insert Size (bp): 696
• Promoter: endogenous GSX2 promoter
• Tag / Fusion Protein:
  • P2A-tGFP (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: ACTGGCCGTCGTTTTAC
• 3′ sequencing primer: CAGGAAACAGCTATGAC

Resource Information

• Supplemental Documents:
  • hGsx2-P2A-tGFP-DTA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry