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GSX2-P2A-tGFP-DTA
(Plasmid #161750)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161750 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GSX2P2Adonor
  • Backbone manufacturer
    Origene
  • Total vector size (bp) 8934
  • Modifications to backbone
    addition of a DTA selection cassette
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    turboGFP
  • Species
    copepod Pontellina plumata
  • Insert Size (bp)
    696
  • Promoter endogenous GSX2 promoter
  • Tag / Fusion Protein
    • P2A-tGFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GSX2-P2A-tGFP-DTA was a gift from Elena Cattaneo (Addgene plasmid # 161750 ; http://n2t.net/addgene:161750 ; RRID:Addgene_161750)

  • For your References section:

    A CRISPR-strategy for the generation of a detectable fluorescent hESC reporter line (WAe009-A-37) for the subpallial determinant GSX2. Besusso D, Cossu A, Mohamed A, Cernigoj M, Codega P, Galimberti M, Campus I, Conforti P, Cattaneo E. Stem Cell Res. 2020 Dec;49:102016. doi: 10.1016/j.scr.2020.102016. Epub 2020 Oct 1. 10.1016/j.scr.2020.102016 PubMed 33039807
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