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pUC57-GSX2-tTA-BleoR
(Plasmid #161748)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161748 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Genescript
  • Total vector size (bp) 4624
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Advanced tTA
  • Species
    Synthetic
  • Promoter endogenous GSX2 promoter

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-GSX2-tTA-BleoR was a gift from Elena Cattaneo (Addgene plasmid # 161748 ; http://n2t.net/addgene:161748 ; RRID:Addgene_161748)

  • For your References section:

    A CRISPR-strategy for the generation of a detectable fluorescent hESC reporter line (WAe009-A-37) for the subpallial determinant GSX2. Besusso D, Cossu A, Mohamed A, Cernigoj M, Codega P, Galimberti M, Campus I, Conforti P, Cattaneo E. Stem Cell Res. 2020 Dec;49:102016. doi: 10.1016/j.scr.2020.102016. Epub 2020 Oct 1. 10.1016/j.scr.2020.102016 PubMed 33039807
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