pSS02:cyt-Peredox-mCherry_DS
(Plasmid
#161747)
-
Purposecytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 161747 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSS02
- Backbone size w/o insert (bp) 9954
- Total vector size (bp) 12723
-
Vector typePlant Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namecyt-Peredox-mCherry_DS
-
SpeciesAequorea victoria; Bacillus subtilis
-
Insert Size (bp)2769
-
Mutationamino acid substitution D115S in first T-Rex domain, amino acid substitution D572S in second T-Rex domain
- Promoter Ubiquitin 10
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 forw
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSS02:cyt-Peredox-mCherry_DS was a gift from Markus Schwarzländer (Addgene plasmid # 161747 ; http://n2t.net/addgene:161747 ; RRID:Addgene_161747) -
For your References section:
In Vivo NADH/NAD+ Biosensing Reveals the Dynamics of Cytosolic Redox Metabolism in Plants. Steinbeck J, Fuchs P, Negroni YL, Elsasser M, Lichtenauer S, Stockdreher Y, Feitosa Araujo E, Kroll JB, Niemeier JO, Humberg C, Smith EN, Mai M, Nunes-Nesi A, Meyer AJ, Zottini M, Morgan B, Wagner S, Schwarzlander M. Plant Cell. 2020 Aug 13. pii: tpc.20.00241. doi: 10.1105/tpc.20.00241. 10.1105/tpc.20.00241 PubMed 32796121