pSS02:cyt-Peredox-mCherry
(Plasmid #161743)

• Purpose: cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants
• Depositing Lab: Markus Schwarzländer
• Publication: Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.00241. doi: 10.1105/tpc.20.00241.

Backbone

• Vector backbone: pSS02
• Backbone size w/o insert (bp): 9954
• Total vector size (bp): 12723
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cyt-Peredox-mCherry
• Species: Aequorea victoria; Bacillus subtilis
• Insert Size (bp): 2769
• Promoter: Ubiquitin 10

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 forw
• 3′ sequencing primer: M13 rev

Resource Information

• Supplemental Documents:
  • pSS02_c-Peredox-mCherry.seq
• A portion of this plasmid was derived from a plasmid made by: the original Peredox-mCherry sequence came from Gary Yellen’s lab via Addgene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry